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July 2001
2002 Santa Cruz Biotechnology, Inc. All Rights Reserved.
Santa Cruz Biotechnology, Inc.
SANTA CRUZ
B I O T E C H N O L O G Y
G
16
(C-18): sc-7415
BACKGROUND
Heterotrimeric G proteins function to relay information from cell
surface receptors to intracellular effectors (1). Each of a very broad
range of receptors specifically detects an extracellular stimulus (a
photon, pheromone, odorant, hormone or neurotransmitter) while
the effectors (i.e., adenylyl cyclase), which act to generate one or
more intracellular messengers, are less numerous. In mammals, G
protein a, b and g polypeptides are encoded by at least 16, 4 and 7
genes, respectively (2-5). Most interest in G proteins has been
focused on their a subunits, since these proteins bind and
hydrolyze GTP and most obviously regulate the activity of the best
studied effectors. Four distinct classes of G subunits have been
identified; these include G
s
, G
i
, unc and G
12/13
(3,4). The G
i
class comprises all the known a subunits that are susceptible to
pertussis toxin modifications, including G
i-1
, G
i-2
, G
i-3
, G
o
,
G
t1
, G
t2
, G
z
and G
gust
(4). Of these, the three G
i
subtypes
function to open atrial potassium channels (6). G
16
is a member
of the G
q
subfamily and is expressed specifically in hematopoietic
cells (7).
SOURCE
G
16
(C-18) is an affinity-purified goat polyclonal antibody
mapping at the carboxy terminus of G
16
of human
origin (identical to corresponding mouse sequence).
PRODUCT
Each vial contains 200 g IgG in 1.0 ml of PBS containing
0.1% sodium azide and 0.2% gelatin.
Blocking peptide is available for competition studies (sc-7415 P)
(100 g peptide in 0.5 ml PBS with 0.1% sodium azide and
100 g BSA).
SPECIFICITY
G
16
(C-18) reacts with G
16
and G
15
of mouse, rat and human
origin by Western blotting and immunohistochemistry.
Recommended dilution range for Western blot analysis:
1:1001 :1000. Recommended starting dilution: 1:100.
STORAGE
Store at 4 C, do not freeze; stable for one year from the date of
shipment.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
REFERENCES
1. Conklin, B.R. and Bourne, H.R. 1993. Structural elements of
G
subunits that interact with G, receptors, and effectors. Cell
73: 631-641.
2. Cali, J.J., Balcueva, E.A., Rybalkin, I., and Robishaw, J.D.
1992. Selective tissue distribution of G protein subunits,
including a new form of the subunits identified by cDNA
cloning. J. Biol. Chem. 267: 24023-24027.
3. McLaughlin, S.K., McKinnon, P.J., and Margolskee, R.F.
1992. Gustducin is a taste-cell-specific G protein closely related to
the transducins. Nature 357: 563-569.
4. Simon, M.I., Strathmann, M.P., and Gautam, N. 1991.
Diversity of G proteins in signal transduction. Science
252: 802-808.
5. von Weizscker, E., Strathman, M.P., and Simon, M.I. 1992.
Diversity among the beta subunits of heterotrimeric GTP-binding
proteins: characterization of a novel beta-subunit cDNA. Biochem.
Biophys. Res. Commun. 183: 350-356.
6. Jones, D.T., Masters, S.B., Bourne, H.R., and Reed, R.R.
1990. Biochemical characterization of three stimulatory
GTP-binding proteins. J. Biol. Chem. 265: 2671-2676.
7. Amatruda, T.T. III, Steele, D.A., Slepak, V.Z., and
Simon, M.I. 1991. G
16
, a G protein alpha subunit specifically
expressed in hematopoietic cells. Proc. Natl. Acad. Sci. USA
88: 5587-5591.
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